BCL2 (18q21.3) Gene Rearrangement
DESCRIPTION – this test is used to detect BCL2 gene rearrangement in B-cell lymphocytes. It’s important to study the BCL2 gene rearrangement for differential diagnosis of non-Hodgkin’s lymphomas. Research has revealed that FISH assay (segregation-based) is the better method as compared to PCR to detect the BCL2 gene mutation as it detects 100% of BCL2 arrangement.
CLINICAL UTILITY – BCL2 is a proto-oncogene, which is originally discovered at the chromosomal breakpoint of the t (14;18) in follicular lymphoma, which regulates apoptosis also known as programmed cell death inhibition of apoptosis may become the cause for the development of tumors.
METHODOLOGY – fluorescence in situ hybridization (FISH)
TURN AROUND TIME – 12 days after sample reaches the lab
PATIENT PREPARATION –
COLLECT
– non diluted bone marrow aspirate
SPECIMEN PREPARATION
– add 3ml of bone marrow to sodium heparin or add 5ml of whole blood
STORAGE OR TRANSPORT TEMPERATURE
– room
temperature
(8
-25 degrees C)
STABILITY
– ambient:
48 hours; refrigerated: 48 hours
UNACCEPTABLE CONDITIONS
–
clotted specimens
MRP – 8000
SPECIAL INSTRUCTIONS – clinical history is mandatory, please bring your previous health reports with you.
![Icon](https://www.cncpathlab.com/assets/images/icons/Get-Reports-in.png)
7 Days
![Icon](https://www.cncpathlab.com/assets/images/icons/age-group.png)
Clinical History is Mandatory