Hepatitis G Virus (HGV) HGV-RNAdetection by PCR

DESCRIPTION - HGV is a single-stranded RNA virus classified in the Flaviviridae family; the virus shares 27 percent homology with hepatitis C virus (HCV). The name HGV denotes two independent viruses: HGV and GBV-C. The HGV has not yet been isolated. The liver is not a significant site of replication.

METHODOLOGY – quantitative polymerase chain reaction

TURN AROUND TIME – 2-5 days

SPECIMEN REQUIERED –

COLLECT – serum separator tube or lavender (EDTA)

SPECIMEN PREPARATION – separate from cells. Transfer 1 ml of serum to transport tube

STORAGE/TRANSPORT TEMPERATURE – frozen

STABILITY – ambient: 24 hours; refrigerated: 1 week; frozen: 1 year

UNACCEPTABLE CONDITION – heparinized specimens

SPECIAL INSTRUCTIONS – clinical history is mandatory