KRAS Mutation Detection Codon 61Tissue

DESCRIPTION – this assay is useful for the detection of KRAS mutation which are associated with shorter progression free survival in colorectal cancer. Codon 12 and codon 13 are more prevalent while codon 61, 117, 146 forms less than 1% of KRAS mutation.

TEST CODE – ME1068

METHODOLOGY – real time polymerase chain reaction (real time PCR)

TURN AROUND TIME – 13 days

PATIENT PREPARATION –

COLLECT – tumor tissue

SPECIMEN PREPARATION – formalin fixed paraffin embedded tissue block. Protect the slides from excessive heat

STORAGE/TRANSPORT TEMPERATURE – room temperature (8-25 degrees C)

STABILITY – ambient: indefinitely; refrigerated: indefinitely

UNACCEPTABLE CONDITIONS – alternate fixatives and decalcified specimens

MRP – 4700

SPECIAL INSTRUCTIONS – clinical history is mandatory