Panfungal-DNA DNA detection byPCR CSF

DESCRIPTION – mutation in the NPM1 gene are involved in a form of blood cell cancer known as cytogenetically normal acute myeloid leukemia

METHODOLOGY – polymerase chain reaction (PCR)

TURN AROUND TIME – 8 days

PATIENT PREPARATION –

COLLECT – lavender (EDTA) or bone marrow

SPECIMEN PREPARATION – transport 5 ml whole blood or 3 ml bone marrow. Specimens must be received within 48 hours of collection due to liability of RNA. Transport 40 micro liter of RNA

STORAGE/TRANSPORT TEMPERATURE – bone marrow or whole blood should be critically refrigerated and extracted RNA should be critically frozen

STABILITY – ambient: 1 hour; refrigerated: 48 hours; frozen: indefinitely (extracted RNA)

UNACCEPTABLE CONDITIONS – serum, plasma, CSF, extracted DNA, clotted specimens

MRP – 3000

SPECIAL INSTRUCTIONS – clinical history is mandatory