Toxoplasma DNA detection by PCRCSF
DESCRIPTION – the presence of toxoplasma DNA in maternal blood probably indicates a recent infection or apparent parasitemia, which is likely to be clinically significant. PCR is the only method that can detect low levels of the T gondii organism and even destroyed parasites
METHODOLOGY – polymerase chain reaction (PCR)
TURN AROUND TIME – 8 days
PATIENT PREPARATION –
COLLECT – lavender, pink or serum separator tube or amniotic fluid, CSF, ocular fluid or tissue
SPECIMEN PREPARATION – separate serum or plasma from cells and transport 1 ml serum, plasma, amniotic fluid, CSF or ocular fluid to a sterile container and transfer tissue to a sterile container and freeze immediately
STORAGE/TRANSPORT TEMPERATURE – frozen
STABILITY –
Tissue – frozen: 3 months
All other – ambient: 8 hours; refrigerated: 5 days; frozen: 3 months
UNACCEPTABLE CONDITION – heparinized specimens
MRP – 2500
SPECIAL INSTRUCTIONS – clinical history is mandatory
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4th day
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2 ml of CSF In Sterile Container